Journal: iScience

Article Title: Single-cell RNA-sequencing of circulating eosinophils from asthma patients reveals an inflammatory signature

doi: 10.1016/j.isci.2025.112609

Figure Lengend Snippet: Healthy human eosinophils have a gene expression profile distinct from other MNCs and granulocytes (A) Experimental schema to obtain scRNA-seq of asthmatic and healthy eosinophil and mononuclear cells. (B) UMAP plot of healthy eosinophils, neutrophils, and mononuclear cells ( n = 51,400). (C) Heatmap of averaged expression of top 500 most significant healthy eosinophil marker genes (Padj <0.05, log2FC > 0.25). Scale represents gene expression Z score. (D) Violin plots of gene expression for canonical eosinophil markers. Adjusted p -values from differential expression testing (Wilcoxon rank-sum test). (E) Heatmap of averaged expression of 154 degranulation pathway genes. Scale represents gene expression Z score.

Article Snippet: Siglec-8 APC , REA1045 , 2 , Eosinophil isolation buffer , 130-117-975 , Miltenyi.

Techniques: Gene Expression, Expressing, Marker, Quantitative Proteomics

Journal: iScience

Article Title: Single-cell RNA-sequencing of circulating eosinophils from asthma patients reveals an inflammatory signature

doi: 10.1016/j.isci.2025.112609

Figure Lengend Snippet: Healthy eosinophils are heterogeneous and vary in expression of essential effector genes (A) UMAP of all eosinophils ( n = 24,538) colored by patient (left) condition (middle) and cluster (right). (B) UMAP of healthy eosinophils only (left) and bar plot of the fraction of healthy cells per cluster (right). (C) Heatmap of average expression of cluster markers (Padj <0.05, log2FC > 0.25). Scale represents gene expression Z score. (D) Top gene ontology biological process pathways enriched in each cluster (Padj <0.05). (E) Violin plots of expression of primary and secondary granule genes and HLA genes. p values were determined by the Kruskal-Wallace test. (F) Dot plot of top interferon-stimulated gene markers for cluster 3 colored by scaled average expression. Adjusted p value from Wilcoxon rank-sum test.

Article Snippet: Siglec-8 APC , REA1045 , 2 , Eosinophil isolation buffer , 130-117-975 , Miltenyi.

Techniques: Expressing, Gene Expression

Journal: iScience

Article Title: Single-cell RNA-sequencing of circulating eosinophils from asthma patients reveals an inflammatory signature

doi: 10.1016/j.isci.2025.112609

Figure Lengend Snippet: Asthmatic eosinophils have distinct gene expression profiles from healthy eosinophils (A) UMAP plot of down sampled eosinophils from healthy and asthma patients colored by condition (left) and cluster (right). (B) Bar plot of fraction of cells per condition for each cluster. (C) Volcano plot of significant DEGs in healthy vs. asthmatic eosinophils (P adj < 0.05, log2FC > 0.25). The top 5 most significant genes per group are labeled. (D) Violin plots of expression of class I HLA genes, antigen presentation genes, granule genes and CCR3. Adjusted p value from Wilcoxon rank-sum test.

Article Snippet: Siglec-8 APC , REA1045 , 2 , Eosinophil isolation buffer , 130-117-975 , Miltenyi.

Techniques: Gene Expression, Labeling, Expressing, Immunopeptidomics

Journal: iScience

Article Title: Single-cell RNA-sequencing of circulating eosinophils from asthma patients reveals an inflammatory signature

doi: 10.1016/j.isci.2025.112609

Figure Lengend Snippet: Asthmatic eosinophils exhibit an inflammatory gene signature (A) GSEA line plots of enriched hallmark pathways in asthma compared to healthy: hallmark interferon gamma response (left), hallmark interferon alpha response (middle), and hallmark inflammatory response (right). (B) Cord plot of 13 genes in the hallmark interferon gamma response, hallmark interferon alpha response, and hallmark inflammatory response pathways which are significantly overexpressed in asthmatic eosinophils compared to healthy.

Article Snippet: Siglec-8 APC , REA1045 , 2 , Eosinophil isolation buffer , 130-117-975 , Miltenyi.

Techniques:

Journal: iScience

Article Title: Single-cell RNA-sequencing of circulating eosinophils from asthma patients reveals an inflammatory signature

doi: 10.1016/j.isci.2025.112609

Figure Lengend Snippet: Mild and severe asthmatic eosinophils have distinct gene expression profiles (A) UMAP clustering of all eosinophils colored by disease severity. (B) Bar plot of fraction of cells per condition for each cluster. (C) Volcano plot of significant DEGs between mild and severe asthmatic eosinophils (P adj < 0.05, log2FC > 0.25). The top 5 most significant genes per group are labeled.

Article Snippet: Siglec-8 APC , REA1045 , 2 , Eosinophil isolation buffer , 130-117-975 , Miltenyi.

Techniques: Gene Expression, Labeling

Journal: iScience

Article Title: Single-cell RNA-sequencing of circulating eosinophils from asthma patients reveals an inflammatory signature

doi: 10.1016/j.isci.2025.112609

Figure Lengend Snippet: Severe asthmatic eosinophils exhibit an inflammatory gene signature (A) Normalized enrichment scores (NES) from GSEA analysis of hallmark gene sets. A negative NES indicates the pathway is enriched in severe eosinophils, and a positive NES indicates the pathway is enriched in mild eosinophils. (B) GSEA line plot (left) and UMAP plot colored by activity score of enriched hallmark interferon alpha response genes (right). (C) GSEA line plot (left) and UMAP plot colored by activity score of enriched hallmark interferon gamma response genes. (D) Selected interferon-stimulated genes which are significantly overrepresented in severe asthmatic eosinophils compared to mild.

Article Snippet: Siglec-8 APC , REA1045 , 2 , Eosinophil isolation buffer , 130-117-975 , Miltenyi.

Techniques: Activity Assay

Journal: Journal of fungi (Basel, Switzerland)

Article Title: Suppression of Chitin-Triggered Immunity by a New Fungal Chitin-Binding Effector Resulting from Alternative Splicing of a Chitin Deacetylase Gene.

doi: 10.3390/jof8101022

Figure Lengend Snippet: Figure 4. Chitin-binding activity of His-tagged PxCDA3 on different chitin oligomers. For these assays, His-tagged proteins were maintained bound to nickel affinity columns and incubated with a mix of chitin oligomers (GlcNAc)1–7. After washing the columns and elution and denaturation of the proteins, the content of oligomers retained by the proteins was analysed. (A) Analysis of chitin oligomers by HPLC. Chromatograms corresponding to reference oligomers are shown in a box on the right. A representative chromatogram from three independent experiments is shown. The peaks corresponding to chitin pentamers and hexamers are indicated. (B) Analysis of chitin oligomers by H-ESI-MS. Representative chromatograms from three independent experiments are shown. The peaks corresponding to chitin pentamers, hexamers, and heptamers are indicated.

Article Snippet: Fungi 2022, 8, 1022 6 of 20 directly analysed using a Jasco high performance liquid chromatography (HPLC) system equipped with a Luna 5 μm NH2 100 Å column (250 × 4.6 mm) (Phenomenex, Madrid, Spain).

Techniques: Binding Assay, Activity Assay, Incubation